eSPC, an Online Data Analysis Platform for Molecular Biophysics

The eSPC platform provides easy to use software for the understanding of biophysical experiments.

Differential Scanning Fluorimetry

Estimating binding affinities by isothermal analysis. Analyse protein-ligand titration curves, fit fluorescence curves, calculate KDs.

Assessing protein stability under different conditions. Calculate Tm, Tonset, DH.

Estimating binding affinities by thermophoresis shift or initial fluorescence analysis.

Estimating molecular masses in a sample.

Estimating sample homogeneity and hydrodynamic radius.

Analysing biomolecules conformation. Perform spectra decomposition, estimate unfolding parameters, or calculate secondary structure content

Burastero, O., Niebling, S., Defelipe, L. A., Günther, C., Struve, A., & Garcia Alai, M. M. (2021). eSPC: an online data-analysis platform for molecular biophysics. Acta Crystallographica Section D: Structural Biology, 77(10).

Niebling, S., Burastero, O., Bürgi, J., Günther, C., Defelipe, L. A., Sander, S., ... & García-Alai, M. (2021). FoldAffinity: binding affinities from nDSF experiments. Scientific reports, 11(1), 1-17.

Bai, N., Roder, H., Dickson, A., & Karanicolas, J. (2019). Isothermal analysis of ThermoFluor data can readily provide quantitative binding affinities. Scientific reports, 9(1), 1-15.

Kotov, V., Mlynek, G., Vesper, O., Pletzer, M., Wald, J., Teixeira‐Duarte, C. M., ... & Marlovits, T. C. (2021). In‐depth Interrogation of Protein Thermal Unfolding Data with MoltenProt. Protein Science, 30(1), 201-217.

Niebling, S., Veith, K., Vollmer, B., Lizarrondo, J., Burastero, O., Schiller, J., ... & García-Alai, M. (2022). Biophysical Screening Pipeline for Cryo-EM Grid Preparation of Membrane Proteins. Frontiers in Molecular Biosciences, 535.

Burastero, O., Draper-Barr, G., Raynal, B., Chevreuil, M., England, P. & Garcia Alai, M. (2023). Raynals, an online tool for the analysis of dynamic light scattering. Acta Crystallographica Section D: Structural Biology, 79(8).