The eSPC platform provides easy to use software for the understanding of biophysical experiments.
Estimate binding affinities by isothermal analysis. Analyse protein-ligand titration curves, fit fluorescence curves, calculate KDs.
Assess protein stability under different conditions. Calculate Tm, Tonset, DH.
Perform a combined analysis of thermal and chemical denaturation. Estimate ΔCp.
Estimate binding affinities by thermophoresis shift or initial fluorescence analysis.
Estimate molecular masses in a sample.
Estimate sample homogeneity and hydrodynamic radius.
Analyse biomolecules conformation. Perform spectra decomposition, estimate unfolding parameters, or calculate secondary structure content
Assess binding kinetics. Perform global fitting of association and dissociation curves, estimate binding rates.
eSPC, a tool for analysing protein biophysics
EMBL (c/o DESY), Notkestrasse 85, Build. 25a, 22607 Hamburg, Germany
Tel: +49 40 89 902-110 or 111