eSPC, an Online Data Analysis Platform for Molecular Biophysics

The eSPC platform provides easy to use software for the understanding of biophysical experiments.

Differential Scanning Fluorimetry

FoldAffinity

FoldAffinity

Estimating binding affinities by isothermal analysis. Analyse protein-ligand titration curves, fit fluorescence curves, calculate KDs.

MoltenProt

MoltenProt

Assessing protein stability under different conditions. Calculate Tm, Tonset, DH.

MicroScale Thermophoresis

ThermoAffinity

ThermoAffinity

Estimating binding affinities by thermophoresis shift or initial fluorescence analysis.

Mass Photometry

PhotoMol

PhotoMol

Estimating molecular masses in a sample.

Dynamic Light Scattering

Raynals

Raynals

Estimating sample homogeneity and hydrodynamic radius.

Circular Dichroism

ChiraKit

ChiraKit (Beta version)

Analysing biomolecules conformation. Perform spectra decomposition, estimate unfolding parameters, or calculate secondary structure content


How to cite

FoldAffinity, MoltenProt & ThermoAffinity

Burastero, O., Niebling, S., Defelipe, L. A., Günther, C., Struve, A., & Garcia Alai, M. M. (2021). eSPC: an online data-analysis platform for molecular biophysics. Acta Crystallographica Section D: Structural Biology, 77(10).

FoldAffinity

Niebling, S., Burastero, O., Bürgi, J., Günther, C., Defelipe, L. A., Sander, S., ... & García-Alai, M. (2021). FoldAffinity: binding affinities from nDSF experiments. Scientific reports, 11(1), 1-17.

Bai, N., Roder, H., Dickson, A., & Karanicolas, J. (2019). Isothermal analysis of ThermoFluor data can readily provide quantitative binding affinities. Scientific reports, 9(1), 1-15.

MoltenProt

Kotov, V., Mlynek, G., Vesper, O., Pletzer, M., Wald, J., Teixeira‐Duarte, C. M., ... & Marlovits, T. C. (2021). In‐depth Interrogation of Protein Thermal Unfolding Data with MoltenProt. Protein Science, 30(1), 201-217.

PhotoMol

Niebling, S., Veith, K., Vollmer, B., Lizarrondo, J., Burastero, O., Schiller, J., ... & García-Alai, M. (2022). Biophysical Screening Pipeline for Cryo-EM Grid Preparation of Membrane Proteins. Frontiers in Molecular Biosciences, 535.

Raynals

Burastero, O., Draper-Barr, G., Raynal, B., Chevreuil, M., England, P. & Garcia Alai, M. (2023). Raynals, an online tool for the analysis of dynamic light scattering. Acta Crystallographica Section D: Structural Biology, 79(8).


Documentation

Code

Our supporters

Core funding

  • European Molecular Biology Laboratory (EMBL)
  • ARISE programme (European Union’s Horizon 2020 research and innovation programme, Marie Skłodowska-Curie grant agreement No 945405)

Grant funding

  • Molecular-scale biophysics research infrastructure (MOSBRI) (European Union’s Horizon 2020 research and innovation programme grant agreement No 101004806)

Workshops & tutorials

About

eSPC, a tool for analysing protein biophysics

Contact

EMBL (c/o DESY), Notkestrasse 85, Build. 25a, 22607 Hamburg, Germany
Tel: +49 40 89 902-110 or 111